Image Source: BD Biosciences, BD Influx Cell Sorter Waste Tank : >Disconnect the Vacuum Pump and Fluidic Lines. >Open Waste Tank and Add 1% Bleach and discard. >Close the lid tightly (To ensure vacuum build up). >Reconnect the vacuum Pump and Fluidics Lines. Sheath Tank : >Release pressure using pressure knob. >Disconnect all the fluid lines connected to the sheath Tank. >Open and check the sheath level (if required, add sheath fluid to the sheath tank). >Close and connect the air and fluid lines. > Connect Sheath Line to Filter to Instrument. Switching ON : >Switch on Condenser, Vacuum Pump, Mains of the Instrument and Computer. >Wait for around 10 minutes to build the pressure in the waste tank and sheath tank. >Wait for 10 minutes for lasers to fully warm up and turn them ON one by one. >Make Sure, the displayed sheath pressure matches the pressure build scale on the sheath tank. >Attach the nozzle to the nozzle asse