Electron Microscopy Image Source: sv.rkriz.net In electron microscope, the source of illumination is electron beam. The construction and principles of electron microscope are easily related to those of light microscope. The range of wavelength of visible light used in light microscope is 4000Å-7000Å, while with an electron microscope employing 60-80KV electron, the wavelength is only 0.05Å. In the instrument, the electron gun generates electron beam. These electrons are concentrated by other components of electron gun producing a fast moving narrow beam of electron. Electrons are focused by electromagnetic lenses. Electromagnetic lens consists of wire encased in soft iron casing. When electric current is passed through the coil, it generates an electromagnetic field through which electrons are focused. There ar...
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Introduction A biophysical technique which is used to study the objects and its areas which cannot be seen through naked eye. (for example: microorganisms like bacteria and yeast and cells like cancer cells & blood cells). A microscope consists of lens, or combination of lenses for making enlarged or magnified images of minute objects. the ability of microscope to detect, resolve and image the smallest items of evidence, often without any alteration or destruction. The aim of any microscope is to magnify images or photos of a small object and to see fine details. There are various types of microscopy. Some of them are bright field microscopy, dark field microscopy, phase contrast microscopy, fluorescent microscopy, electron microscopy, transmission electron microscopy and scanning electron microscopy Light Microscope or Compound Microscope: Image Source: www.microscope.com A compound microscope has two set lenses. One is objective lens and other one is a...