BIOMAG

Image Source: genomecore.ucsf.edu Real Time PCR is a molecular biology instrument which is able to monitor the amplification of the target or template DNA at each cycle. Hence the name "Real Time PCR".. It can also be called as quantitative PCR as it is able to quantify the template DNA. It works on the basis of polymerase chain reaction in addition to that it utilizes the fluorescent dyes, fluorescent reporters or probes to monitor the amplification of template in DNA by emitting the fluorescence at each cycle.  There are two main methods for the detection of amplification of DNA in real time.  1) Non-specific fluorescent dyes such as SYBR Green Dye which binds to any double-stranded DNA present in the PCR tube.  2) Sequence-specific DNA Probes consisting of oligo nucleotides which are labelled with fluorescent reporters.  Example: TaqMan. Principle of Real-Time PCR :     "A thermal cycler which is installed with an illuminator wh...

                         A PCR (Polymerase Chain Reaction) is one of the great and most widely used to technique in molecular biology. and has wide range of applications in medical and clinical laboratories for diagnosis purposes and this technique is widely and most commonly used in biological or bio medical research.  But, there are many types of PCR which have different applications and different levels of sensitivity. Some of the types of PCR are listed below with brief explanation.  Ligation-Mediated PCR : uses small DNA oligo nucleotide linkers (or adaptors) which are first ligated to DNA fragments of the target or template DNA.  Reverse Transcription PCR:  a reverse transcription enzyme called "reverse transcriptase" which will amplify the RNA strand to DNA strand called cDNA (complementary DNA). In simple terms, this type of PCR generates complementary DNA Strands from a t...

             PCR stands for "Polymerase Chain Reaction" in biological sciences, especially in molecular biology. A molecular biology technique which makes millions of copies of a target DNA. A target single copy of DNA molecule is exponentially amplified which produces millions of copies of that particular target DNA segment with the help of primers, enzymes and nucleotides.       PCR has wide range of applications in molecular biology.. This technique has been regularly used in clinical and medical laboratories, in biological or biomedical research and forensic sciences (crime investigation.  Most of PCR works on the principle of Thermal Cycling. Thermal Cycling is a process where the DNA molecules and constituents along with that segment is repeatedly heated and cooled.  Principle of PCR :                     "In the first step, the target DNA segment is heated...