BIOMAG

Fluorescence Microscopy and Flow Cytometry, both are biological instruments which are capable of measuring the biophysical and biochemical properties of cells or particles which are under study. Both of them are capable of identifying types of cells present in a given sample. These two technologies are useful in variety of applications and they have widespread usage... But, These two differ in their applications and use.  Fluorescence Microscopy Flow Cytometry Sample mounted on a glass slide. Sample is allowed to pass through a liquid stream through a sensing area. (Single cell suspension). Produces magnified images of the cells Does not produce the images. Whole Tissue section can be visualized and analyzed Tissue must be disintegrated into single cell suspension. And each single cell is analyzed. Detection through human eye and images can be produced on a computer screen Detection is e

Please Watch The Demo Video attached after the protocol and example Data!!!! Preparation of Samples for Cell Cycle using Flow Cytometry :  1) Suspend approximately million cells (10^6) in 1mL of 1x PBS (phosphate-buffered saline solution). and with the help of pipette, gently aspirate (up and down) about 7-8 times, to make the single cell suspension.  2) Take a centrifuge tube and add 4mL of 70% ethanol.. (70% ethanol should be in ice before using). and add 1mL of cells in PBS to the same tube. Keep the tubes on ice for minimum 2 hours. This step is to fix the cells. (Cells can be kept at 4℃ for a week). 3) Centrifuge the ethanol fixed cells for 5 minutes at 300g. Carefully take out the supernatant. Do not disturb the pellet.  4) Add 5mL of cold 1xPBS to this pellet and suspend the cells using pipette.  5) Centrifuge the tubes at 300g for 5 minutes. Discard the supernatant. Again do not disturb the pellet.  6) If required, step 5 can be repeated f

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