Job opening : Indian Institute of Science

INDIAN INSTITUTE OF SCIENCE, BANGALORE

ADVERTISEMENT

The Indian Institute of Science (IISC), a leading institution for research in science and engineering, runs an integrated Ph.D. Programme for several years now.
Details of the Programme can be found at the Institute web site http://www.iisc.ac.in/

In this context, IISc invites application for the position of one Project Assistant for the Integrated Ph.D Programme to help in conducting laboratory courses of
this program in the discipline of Chemistry.

As eligibility, an applicant for the position of Project Assistant should possess M.Sc. in Chemistry, preferably in organic or inorganic and physical chemistry
specialization, with a passion for teaching, especially laboratory instruction.

The Project Assistant position in on a Contractual basis and the candidate will be hired on a renewable (for satisfactory performance) annual contract, with a
fixed salary based on experience up to a maximum duration of 5 years. The salary of a Project Assistant will be a lump-sum of Rs. 18,000 to Rs. 25,000.

Job Description:
The Project Assistant work involves setting up of experiments, maintenance of the experimental set-ups and general infrastructure of the laboratory in
consultation with the Int. Ph.D Instructor and the Division faculty. The project Assistant would also be needed to assist in the conduct of tutorial classes and
course material preparation (both print and electronic versions). Further, he/she would help the convener in the general administration of the Programme.

Model of Selection:

The selection of the candidates will be on the basis of their CV and performance in an interview to be conducted at the Indian Institute of Science, Bangalore.
The soft copy of CV/Application (current CV, account of past experience if any, statement of interest) should be sent by electronic mail to nravi@iisc.ac.in
addressed to: the Convener, Int. Ph.D Programme, Materials Research Centre, Indian Institute of Science, Bangalore-560012.

Please mention in the e-mail subject line the position applied for and do not send any hard copy of your application.

The last date of receiving the applications is 30 January 2019

Short-listed candidates will be called for an interview subsequently.

Comments

Statistics in Flow Cytometry Data and "MFI" values

The speed of the flow cytometry offers wide range of data points and data plots. Due to its sensitivity and versatility, it has been used extensively. Flow cytometer can detect up to 1000s of cells per second.. But, it should be noted that the flow cytometry also involves the statistics, its significance, calculating the fluorescence intensity.. Statistics in flow cytometry involves, total number of data points acquired, percentage population, most importantly mean and median fluorescence intensity, and others. Most of us get confused about the term "MFI", whether it means mean fluorescence intensity or median fluorescence intensity.. Truth is, both.. It means it can be described as either mean or median fluorescence intensity. Some researchers use Mean for MFI and some use Median for MFI values. (Keep...

Fluorescence Spectrum Viewers Tool.

Spectra Viewers are the fluorochrome tools which help in understanding the wavelength ranges of excitation and emission of fluorochromes.. These tools are very helpful in determining specific laser, detectors and range of fluorescence spectra. When Multi-Color Flow Cytometry is the experiment, then these must be used to analyze emission spectrum, fluorescence spill over and compensation values. How these tools help.. First, Lets consider a simple example of analyzing the spectrum for Green Fluorescence Protein (GFP) and you want to know the excitation wavelength and emission wavelength range for GFP. By knowledge, you know that GFP will excite with 488nm Blue laser and emits the green fluorescence at apporximately 510nm.. Now, just open a spectrum viewer and select the GFP as shown below. Now, you have analyzed GFP fluorescence and received the knowledge that It excites with 488nm Blue Laser and emission maximum is...

Compensation in Flow Cytometry (Fluorescence Spill Over Correction).

The term "Compensation" in flow cytometers refers to the process of correcting fluorescence spill over. The compensation matrix ensures that the fluorescence emitted is detected in a particular detector which is from the fluorescence being measured. As we all know that most of the fluorescence molecules have broad emission range. if more than one one fluorescence is selected, their emission spectra may overlap. To correct this spectral overlap in cytometry data, a mathematical matrix called compensation is used. The Intention of this article is to teach you the compensation from basic theory to practical. This example for FITC and PE fluorescence molecules shows that, Some of the FITC fluorescence is being detected in PE Detector. and Some of the PE fluorescence is being detected in FITC Detectors. Task here is to make sure that in cytometry data, FITC is in FITC detector only and PE is in PE Detector only....

BIOMAG

Search This Blog